كيف تشكّل القطرات داخل الخلايا الأغشية سريع

الملخص الأصلي

كما كتبه المؤلفون، بالإنجليزية arXiv:2603.15904

Phase-separated biomolecular condensates with liquid-like properties play a key role in the organization and compartmentalization of the intracellular environment. Condensate-mediated capillary forces acting on membranes drive physiologically important reshaping of membrane-bound organelles, such as vacuoles and autophagosomes. Here, we explore condensate-mediated membrane shape transformations. We employ {\textit{in planta}} live-cell imaging, an \textit{in vitro} reconstitution system with tunable interfacial tension, and computer simulations of an elastic membrane model to describe three morphologies of membrane structures localized at condensate interfaces: tubes, sheets, and cups. We find that the forces associated with high interfacial tension drive the formation of stable sheets, while tubes and cups prevail at lower interfacial tension. We calculate the free energies of each membrane shape and identify the energy barriers that govern the transitions between the shapes. With this approach, we find that shape transformations depend on the history of the interfacial membrane and exhibit a tube-to-cup hysteresis. These findings indicate that temporal control of condensate surface properties can mediate the morphogenesis of cup-like structures in cells, such as the formation of "bulbs" within plant vacuoles. Our results further generalize how the interplay of condensates and membranes contributes to intracellular organization.