
A technique that observes how light scatters to identify molecular vibrations and composition.Raman spectroscopy is a technique that probes molecular vibrations by analyzing how light scatters inelastically. When a photon hits a molecule, most of it scatters elastically (Rayleigh scattering) with the same energy, but a tiny fraction exchanges energy with the molecule's vibrational or rotational modes, shifting the photon's wavelength. This shift, called the Raman effect, provides a unique 'fingerprint' of the molecular structure and composition, making it a powerful tool in chemistry, biology, and materials science.
The effect was predicted by Adolf Smekal in 1923 and experimentally observed by C.V. Raman and K.S. Krishnan in 1928. Raman received the Nobel Prize in Physics in 1930 for this discovery.
A laser beam illuminates a sample, and the scattered light is collected and analyzed with a spectrometer. The Raman spectrum shows peaks at frequency shifts corresponding to specific molecular vibrations. The intensity and position of these peaks reveal the chemical bonds, crystal structure, and even stress or temperature in the material. Because Raman scattering is inherently weak (about one in a million photons), modern systems use high-power lasers, sensitive detectors, and techniques like surface-enhanced Raman scattering (SERS) to amplify the signal.
Raman spectroscopy is used in pharmaceutical quality control, forensic analysis, art conservation, and biomedical diagnostics. It is also a key tool in planetary science, such as on NASA's Perseverance rover, which uses it to detect organic compounds on Mars.
Raman spectroscopy can identify the composition of a single cell or a grain of dust without damaging it, which is why it is used to analyze precious artifacts and even the contents of ancient pottery.


