Techniques for measuring temperature at microscopic scales, such as within cells or at material interfaces.Nanoscale temperature measurement refers to techniques that determine temperature with spatial resolution on the order of nanometers, far beyond the reach of conventional thermometers. This is crucial because temperature can vary dramatically over tiny distances — for example, inside a living cell, at the junction of two materials, or within a microelectronic device. Knowing the local temperature is essential for understanding heat flow, chemical reactions, and the stability of nanoscale systems.
The field emerged in the 1990s with the development of scanning thermal microscopy and fluorescent nanothermometry, driven by the need to measure heat in microelectronics and biological systems.
Most methods rely on temperature-dependent properties of tiny probes. Fluorescent nanoparticles (nanothermometers) change their emission spectrum or intensity with temperature; when attached to a cell or a surface, their glow reports the local temperature. Alternatively, scanning probe techniques use a sharp tip that measures thermal expansion or heat flow between the tip and the sample, achieving nanometer resolution. Other approaches use the temperature-dependent resistance of nanowires or the frequency shift of vibrating nanostructures.
It is used in biology to measure heat production in single cells, in electronics to find hot spots in microchips, and in nanomedicine to monitor hyperthermia treatments.
Some nanothermometers are so small they can be placed inside a single organelle within a cell, as suggested by the article 'Лазер размером с органеллу'.
| D | variable | Diffusion coefficient — diffusion coefficient metre squared per second |
| E | variable | Particle energy — energy joule |
| B | variable | Magnetic field strength — magnetic field strength tesla |
| R_inj | variable | Injection scale (turbulence outer scale) — length metre |








