
The chemical reaction that breaks water molecules into oxygen and hydrogen, a key step in photosynthesis.Water splitting is the chemical reaction that breaks water molecules (H₂O) into oxygen (O₂) and hydrogen (H₂). In nature, this is the heart of photosynthesis, where plants use sunlight to power the reaction, releasing the oxygen we breathe and storing energy in chemical bonds. The process is also central to the emerging field of artificial photosynthesis, which aims to produce clean hydrogen fuel directly from water and sunlight.
The discovery that water can be split by light was made in 1789 by Jan Ingenhousz, who showed that plants produce oxygen in sunlight. The detailed mechanism of the photosynthetic water-splitting complex was only fully mapped in the 21st century, with the crystal structure of Photosystem II solved in 2011.
The reaction is endothermic, meaning it requires an energy input. In photosynthesis, light energy is absorbed by pigments and used to strip electrons from water molecules at a catalytic site containing manganese and calcium. This releases protons and oxygen gas. The electrons are then shuttled through a chain of carriers to drive further reactions. In artificial systems, the same principle is applied: a catalyst lowers the energy barrier, and an external energy source (light or electricity) drives the separation of water into its components.
Water splitting is used in electrolyzers to produce hydrogen fuel, and in artificial photosynthesis research to create solar fuels. It is also the basis of the oxygen-evolving cycle in all oxygenic photosynthetic organisms.
The oxygen we breathe comes from water, not from carbon dioxide—a fact established by C.B. van Niel in the 1930s using isotopic labeling.














