
The process where a mixture separates into distinct regions of different composition.Phase separation is a phenomenon where a homogeneous mixture spontaneously splits into distinct regions with different compositions, like oil and water separating into layers. This process is driven by thermodynamics: the system lowers its free energy by segregating components. It occurs in a wide range of contexts, from the formation of lipid rafts in cell membranes to the demixing of alloys during solidification.
The thermodynamic theory of phase separation was developed in the late 19th century by Gibbs, with later contributions by Cahn and Hilliard in the 1950s for spinodal decomposition.
When the interactions between different components are unfavorable (e.g., repulsive), mixing becomes energetically costly. At certain temperatures and concentrations, the system becomes unstable, and small fluctuations in composition grow, leading to the nucleation and growth of domains. This can happen via spinodal decomposition, where the mixture spontaneously separates everywhere, or via nucleation, where droplets form and grow. The final pattern depends on the balance of diffusion and interfacial energy.
Phase separation is exploited in materials science to create alloys with tailored properties, in biology to understand cellular organization, and in industry for processes like polymer blending and protein purification.
In cells, phase separation creates membraneless organelles like stress granules, which act as tiny reaction vessels, and their malfunction is linked to neurodegenerative diseases.










