Spectroscopy using nitrogen-vacancy centers in diamond for biological and magnetic sensing applications.Diamond NV center spectroscopy exploits the nitrogen-vacancy (NV) center — a point defect in diamond where a nitrogen atom sits next to a vacant lattice site — as a quantum sensor. The NV center's electronic spin state is sensitive to magnetic fields, electric fields, temperature, and strain, and can be read out optically. This makes it a versatile probe for nanoscale measurements in biology, materials science, and quantum technology.
The NV center's spin properties were first characterized in the 1990s, but its use as a practical sensor gained momentum in the 2000s with demonstrations of single-spin magnetic resonance and nanoscale imaging.
A green laser excites the NV center, and its red fluorescence intensity depends on the spin state, which is manipulated with microwave pulses. By measuring the fluorescence as a function of microwave frequency, one obtains an optically detected magnetic resonance (ODMR) spectrum. Shifts in the resonance frequencies reveal local magnetic fields, temperature changes, or stress with high precision, even at the single-defect level.
Used for high-resolution magnetic imaging of biological cells, mapping temperature gradients inside living organisms, and sensing magnetic fields in spintronic devices.
A single NV center in diamond can detect the magnetic field of a single electron spin at room temperature.


















