A method using focused laser beams to trap and manipulate microscopic particles.Optical tweezers are a technique that uses a highly focused laser beam to trap and manipulate microscopic objects, such as individual cells, bacteria, or even single molecules. The gradient force of the light field pulls the particle toward the focal point, holding it in place. This tool has revolutionized biology and physics by allowing researchers to hold, move, and measure forces on tiny objects with unprecedented precision.
Arthur Ashkin developed optical tweezers in 1970 at Bell Labs, for which he received the Nobel Prize in Physics in 2018. His early work demonstrated trapping of dielectric spheres and later biological particles.
A laser beam is focused through a high-numerical-aperture microscope objective. The electric field gradient creates a force that attracts dielectric particles toward the region of highest intensity. The scattering force pushes the particle along the beam direction, but the gradient force dominates near the focus, creating a stable trap. By moving the beam or the sample stage, the trapped particle can be manipulated. The stiffness of the trap can be calibrated to measure piconewton forces.
Optical tweezers are used to study molecular motors, DNA mechanics, cell sorting, and to assemble microstructures. They are also used in quantum optics to trap neutral atoms.
Optical tweezers can trap and manipulate individual atoms, which was key to many quantum computing experiments.























