
A range of light wavelengths that passes through biological tissue with minimal loss, used for in-cell applications.Near-infrared light occupies the part of the electromagnetic spectrum just beyond visible red, with wavelengths roughly from 700 nanometers to a few micrometers. In biological tissue, this range is special: water and hemoglobin absorb visible light strongly, but near-infrared photons scatter less and encounter fewer absorbing molecules, allowing them to penetrate several centimeters deep. This 'optical window' makes near-infrared the go-to choice for non-invasive imaging and sensing inside living organisms, from pulse oximeters to brain activity monitors.
The optical window was recognized in the mid-20th century, with systematic studies by researchers like Britton Chance in the 1970s–80s who applied near-infrared spectroscopy to measure tissue oxygenation.
The key is that tissue components like water, lipids, and oxy- and deoxy-hemoglobin have low absorption coefficients in the near-infrared range, while scattering is relatively weak compared to visible light. Photons in this window undergo many random scattering events but still travel far enough to return to a detector, carrying information about absorption changes caused by blood flow or oxygen levels. In microscopy, near-infrared excitation also reduces photodamage and autofluorescence, enabling long-term observation of living cells.
Used in pulse oximeters, functional near-infrared spectroscopy (fNIRS) for brain imaging, and deep-tissue fluorescence microscopy.
Near-infrared light can pass through several centimeters of tissue, which is why it is used to measure oxygen levels in a baby's brain during birth.















